Matthias Stadtfeld, PhD

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Assistant Professor of Cell Biology PhD,
2005, Albert Einstein College of Medicine

LAB WEBSITE:
Stadtfeld Lab Website
KEYWORDS:
Induced pluripotency, reprogramming, imprinting, hematopoietic stem cells

Contact Information

Skirball Institute of Biomolecular Medicine
Developmental Genetics Program
New York University School of Medicine
540 First Ave. 4th floor
New York, NY 10016
E-mail: Matthias.Stadtfeld@nyumc.org


Molecular and epigenetic mechanisms that control the fate of embryonic and adult stem cells

 

Pluripotent cells that have the unique ability to form all cell types of the adult body can be derived in two different ways: 1) by explanting early mammalian embryos, thereby giving rise to embryonic stem (ES) cells and 2) by the enforced expression of defined embryonic transcription factors in adult somatic cells, giving rise to induced pluripotent stem (iPS) cells.  The latter process is commonly referred to as reprogramming and allows for the comparatively straightforward generation of patient-specific pluripotent stem cells to study, and ultimately possibly treat, degenerative disorders. In addition, iPSC technology represents a tractable experimental approach to study mammalian development.  

In addition to working towards a better understanding of the cellular and molecular mechanisms that allow the induction of pluripotency in essentially any somatic cell type, my laboratory uses reprogramming technology as a tool to probe the epigenetic regulation of gene loci that are subject to genomic imprinting. For this, we are using the study of genetically engineered mouse models as our primary experimental approach. We are especially interested in the reasons for the observed instability of genomic imprinting in certain pathological and possibly physiological conditions and in the consequences of their dysregulation for the organism. A second major goal of the laboratory is focused on identifying molecular roadblocks for the specification of blood cells in the mammalian embryo as well as in cell culture. Ultimately, we would like to use pluripotent cells for the in vitro generation of blood stem and progenitor cells that are functionally equivalent to their in vivo counterparts.

Selected Publications: 

Stadtfeld M*, Apostolou E* et al., (2012) Ascorbic acid prevents loss of Dlk1-Dio3 imprinting and facilitates generation of all-iPS cell mice from terminally differentiated B cells. Nature Genetics. Mar 4;44(4): 398-405. PMID: 22387999

Stadtfeld M and Hochedlinger K. (2010) Induced pluripotency: history, mechanisms & applications. Genes Dev. Oct; 24: 2239-63. PMID: 20952534

Stadtfeld M*, Apostolou E*, Akutsu H, Fukuda A, Follett P, Natesan S, Kono T, Shioda T, Hochedlinger K. (2010) Aberrant silencing of imprinted genes on chromosome 12qF1 in mouse induced pluripotent stem cells. Nature. May 13;465(7295):175-81.* equal contribution. PMID: 20418860

Stadtfeld M, Nagaya M, Utikal J, Weir G, Hochedlinger K. (2008) Induced Pluripotent Stem Cells Generated Without Viral Integration. Science. Nov 7;322(5903):945-9. PMID: 18818365

Stadtfeld M, Maherali N, Breault DT, Hochedlinger K. (2008) Defining molecular cornerstones during fibroblast to iPS cell reprogramming in mouse. Cell Stem Cell, Mar 6;2 (3):230-40. PMID: 18371448